Review



human embryonic kidney hek 293t parent cell lines  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC human embryonic kidney hek 293t parent cell lines
    Human Embryonic Kidney Hek 293t Parent Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 38264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+embryonic+kidney+hek+293t+parent+cell+lines/293T/pmc12571082-214-3-14
    Average 99 stars, based on 38264 article reviews
    human embryonic kidney hek 293t parent cell lines - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: A CRISPR activation screen reveals a cilia disassembly pathway mutated in focal cortical dysplasia.
    Article Snippet: Where specified, cells were treated with 250 nM SAG (Enzo Life Sciences), 6 μM vorapaxar (VWR), 30 μM DSRM- 3716 (Tocris), 10 μM dHNN (Toronto Research Chemicals), 5 or 10 μM dantrolene (as indicated, Tocris), 10 μM Y- 27632, 5 μM BAPTA- AM, 2 μM tubacin, 100 nM CytoD (SigmaAldrich), 15 μM LPA (Sigma- Aldrich), 250 nM Torin1 (APExBIO), bovine thrombin (10 U/ml; Sigma- Aldrich), or Dox (0.01 to 1.0 μg/ml; Thermo Fisher Scientific).

    Article Title: A CRISPR activation screen reveals a cilia disassembly pathway mutated in focal cortical dysplasia
    Article Snippet: NIH-3T3, RPE1, and human embryonic kidney (HEK) 293T parent cell lines were obtained from American Type Culture Collection; NIH-3T3 FlpIn cells were provided by D. Mick (Saarland University).



    Similar Products

    human embryonic kidney hek 293t parent cell lines  (ATCC)
    99
    ATCC human embryonic kidney hek 293t parent cell lines
    Human Embryonic Kidney Hek 293t Parent Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+embryonic+kidney+hek+293t+parent+cell+lines/293T/pmc12571082-214-3-14
    Average 99 stars, based on 1 article reviews
    human embryonic kidney hek 293t parent cell lines - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    parent hek 293t cell line human embryonic kidney hek 293t cells  (ATCC)
    99
    ATCC parent hek 293t cell line human embryonic kidney hek 293t cells
    <t>HEK</t> <t>293T</t> cells expressing 10 different class II SLA α and β heterodimers in the presence (black bars) or absence (red bars) of pig CD74 were tested for: (A) Binding to a monoclonal antibody, specific for class II SLA and (B) Binding of CerCLIP.1, a monoclonal antibody specific for invariant chain peptides associated with class II SLA. CD74 expression significantly increased antibody binding (p=0.002 for both assays, Wilcoxon matched-pairs sign rank test). Specific alleles making up each alpha-beta combination are indicated in table 2. DR indicates SLA-DRα/DRβ pairs, DQ represents SLA-DQα/DQβ pairs, and hybrid represent SLA-DRα/DQβ pairs.
    Parent Hek 293t Cell Line Human Embryonic Kidney Hek 293t Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+embryonic+kidney+hek+293t+parent+cell+lines/293T/pmc05978423-67-3-14
    Average 99 stars, based on 1 article reviews
    parent hek 293t cell line human embryonic kidney hek 293t cells - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    HEK 293T cells expressing 10 different class II SLA α and β heterodimers in the presence (black bars) or absence (red bars) of pig CD74 were tested for: (A) Binding to a monoclonal antibody, specific for class II SLA and (B) Binding of CerCLIP.1, a monoclonal antibody specific for invariant chain peptides associated with class II SLA. CD74 expression significantly increased antibody binding (p=0.002 for both assays, Wilcoxon matched-pairs sign rank test). Specific alleles making up each alpha-beta combination are indicated in table 2. DR indicates SLA-DRα/DRβ pairs, DQ represents SLA-DQα/DQβ pairs, and hybrid represent SLA-DRα/DQβ pairs.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Examining the Biosynthesis and Xenoantigenicity of Class II SLA Proteins

    doi: 10.4049/jimmunol.1800022

    Figure Lengend Snippet: HEK 293T cells expressing 10 different class II SLA α and β heterodimers in the presence (black bars) or absence (red bars) of pig CD74 were tested for: (A) Binding to a monoclonal antibody, specific for class II SLA and (B) Binding of CerCLIP.1, a monoclonal antibody specific for invariant chain peptides associated with class II SLA. CD74 expression significantly increased antibody binding (p=0.002 for both assays, Wilcoxon matched-pairs sign rank test). Specific alleles making up each alpha-beta combination are indicated in table 2. DR indicates SLA-DRα/DRβ pairs, DQ represents SLA-DQα/DQβ pairs, and hybrid represent SLA-DRα/DQβ pairs.

    Article Snippet: 2.2 Culture of Parent HEK 293T Cell Line Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in minimum essential media (MEM- α) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) (HyClone, Logan UT) in collagen-I-coated plates (Becton Dickinson, Bedford, MA) at 37°C and 5% CO 2 .

    Techniques: Expressing, Binding Assay

    Cell lines expressed CD74, SLA-DQα1*0101, and mutant or unmodified SLA-DQβ1*0601. Mutant molecules contained a β chain having proline at position 55 (55P). Wild type class II contained arginine at position (55R). (A) White histograms show binding of the CerCLIP.1 antibody to cells expressing either 55R or 55P. Gray histograms show CerCLIP.1 binding to CD74/SLA-deficient HEK 293T cells. (B) 21 human sera were incubated with cells expressing either the 55R or 55P variants of SLA-DQ. These sera were selected from prior experiments suggesting they reacted with SLA-DQα1*0101/SLA-DQβ1*0601. Data from each sample is represented by one red and one black bar (stacked on top of each other). MFI ratios of fluorescent anti-human IgG were calculated to compare binding to 55P versus 55R molecules. The log10 values of these ratios were plotted for each serum (black bars). The same analysis compared IgG binding to the 55P cells versus SLA-negative parent cells (red bars). The 55P mutation partially reduced IgG binding to the SLA-DQ protein in 17 samples. Three samples had no detectable antibody staining of 55P mutants (#). One sample showed greater binding to the 55P mutant than to 55R (*). The mAB sample indicates the binding of a monoclonal antibody specific for SLA-DQ. This antibody recognized SLA-DQ 55P variants (red bar) almost identically to 55R variants (black bar, Log10 of 55P/55R = −0.09).

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Examining the Biosynthesis and Xenoantigenicity of Class II SLA Proteins

    doi: 10.4049/jimmunol.1800022

    Figure Lengend Snippet: Cell lines expressed CD74, SLA-DQα1*0101, and mutant or unmodified SLA-DQβ1*0601. Mutant molecules contained a β chain having proline at position 55 (55P). Wild type class II contained arginine at position (55R). (A) White histograms show binding of the CerCLIP.1 antibody to cells expressing either 55R or 55P. Gray histograms show CerCLIP.1 binding to CD74/SLA-deficient HEK 293T cells. (B) 21 human sera were incubated with cells expressing either the 55R or 55P variants of SLA-DQ. These sera were selected from prior experiments suggesting they reacted with SLA-DQα1*0101/SLA-DQβ1*0601. Data from each sample is represented by one red and one black bar (stacked on top of each other). MFI ratios of fluorescent anti-human IgG were calculated to compare binding to 55P versus 55R molecules. The log10 values of these ratios were plotted for each serum (black bars). The same analysis compared IgG binding to the 55P cells versus SLA-negative parent cells (red bars). The 55P mutation partially reduced IgG binding to the SLA-DQ protein in 17 samples. Three samples had no detectable antibody staining of 55P mutants (#). One sample showed greater binding to the 55P mutant than to 55R (*). The mAB sample indicates the binding of a monoclonal antibody specific for SLA-DQ. This antibody recognized SLA-DQ 55P variants (red bar) almost identically to 55R variants (black bar, Log10 of 55P/55R = −0.09).

    Article Snippet: 2.2 Culture of Parent HEK 293T Cell Line Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in minimum essential media (MEM- α) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) (HyClone, Logan UT) in collagen-I-coated plates (Becton Dickinson, Bedford, MA) at 37°C and 5% CO 2 .

    Techniques: Mutagenesis, Binding Assay, Expressing, Incubation, Staining

    7 sera lacking class II HLA antibodies and 7 sera containing class II HLA antibodies were incubated with cells expressing pig CD74 and individual combinations of class II SLA alleles. Table 2 describes the class II allele combinations. Parent cells were HEK 293T cells devoid of CD74 and class II SLA. Rabbit complement was added and dead cells counted as an indicator of antibody initiated complement cytotoxicity (percent killing). (A) Cytotoxicity of sera lacking class II HLA antibodies. (B) Cytotoxicity of sera containing class II HLA antibodies. DTT was added to inactivate IgM in panels C (sera lacking class II HLA antibodies) and D (sera containing class II HLA antibodies). DR indicates SLA-DRα/DRβ pairs, DQ represents SLA-DQα/DQβ pairs, and H represent SLA-DRα/DQβ pairs. Parent represents killing against cells lacking both class II SLA proteins and CD74. Panel E shows the reduction of cell killing for a single serum against cells expressing all 10 class II SLA proteins before and after DTT treatment (±DTT comparison p = 0.001, Wilcoxon test).

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Examining the Biosynthesis and Xenoantigenicity of Class II SLA Proteins

    doi: 10.4049/jimmunol.1800022

    Figure Lengend Snippet: 7 sera lacking class II HLA antibodies and 7 sera containing class II HLA antibodies were incubated with cells expressing pig CD74 and individual combinations of class II SLA alleles. Table 2 describes the class II allele combinations. Parent cells were HEK 293T cells devoid of CD74 and class II SLA. Rabbit complement was added and dead cells counted as an indicator of antibody initiated complement cytotoxicity (percent killing). (A) Cytotoxicity of sera lacking class II HLA antibodies. (B) Cytotoxicity of sera containing class II HLA antibodies. DTT was added to inactivate IgM in panels C (sera lacking class II HLA antibodies) and D (sera containing class II HLA antibodies). DR indicates SLA-DRα/DRβ pairs, DQ represents SLA-DQα/DQβ pairs, and H represent SLA-DRα/DQβ pairs. Parent represents killing against cells lacking both class II SLA proteins and CD74. Panel E shows the reduction of cell killing for a single serum against cells expressing all 10 class II SLA proteins before and after DTT treatment (±DTT comparison p = 0.001, Wilcoxon test).

    Article Snippet: 2.2 Culture of Parent HEK 293T Cell Line Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in minimum essential media (MEM- α) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) (HyClone, Logan UT) in collagen-I-coated plates (Becton Dickinson, Bedford, MA) at 37°C and 5% CO 2 .

    Techniques: Incubation, Expressing, Comparison